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Isolation and characterization of highly purified mosquito oostatic hormone
Author(s) -
Borovsky Dov
Publication year - 1985
Publication title -
archives of insect biochemistry and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.576
H-Index - 66
eISSN - 1520-6327
pISSN - 0739-4462
DOI - 10.1002/arch.940020402
Subject(s) - aedes aegypti , biology , vitellogenin , size exclusion chromatography , hormone , vitellogenesis , juvenile hormone , yolk , ovary , chromatography , biochemistry , endocrinology , chemistry , oocyte , larva , embryo , enzyme , microbiology and biotechnology , botany , ecology , gene
Oostatic hormone, the hormone that inhibits vitellogenesis in mosquitoes, was purified 7,000‐fold with a recovery of 70% from the ovaries of the mosquito Aedes aegypti . The purification procedure included heat treatment and chromatography on ion exchange and gel filtration columns. The hormone is a small peptidelike molecule of molecular weight 2,200 at pH 4.5, which aggregates into larger molecular species of trimer and octamer at pH 7.0 as determined by gel filtration. The hormone is positively charged at pH 7.8 and has a low Rf at pH 9.4 on disc gel electrophoresis. Injection of purified oostatic hormone (9 ng) into female mosquitoes inhibited yolk deposition and vitellogenin synthesis. Activity of the oostatic hormone in the mosquito ovary increased rapidly following blood feeding and reached a maximum after 48 h. Oostatic hormone of A. aegypti injected into autogenous Aedes taeniorhynchus inhibited egg development. Repeated injections of dilute oostatic hormone at 24 h intervals partially arrested egg development, resulting in 60% reduction in the number of eggs laid. This hormone does not block release of egg development neurosecretory hormone (EDNH) from the mosquito brain but rather appears to act on the ovary.