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New ecdysteroid conjugate: Isolation and identification of 26‐hydroxyecdysone 26‐phosphate from eggs of the tobacco hornworm, Manduca sexta (L.)
Author(s) -
Thompson Malcolm J.,
Weirich Gunter F.,
Rees Huw H.,
Svoboda James A.,
Feldlaufer Mark F.,
Wilzer Kenneth R.
Publication year - 1985
Publication title -
archives of insect biochemistry and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.576
H-Index - 66
eISSN - 1520-6327
pISSN - 0739-4462
DOI - 10.1002/arch.940020302
Subject(s) - manduca sexta , ecdysteroid , conjugate , 20 hydroxyecdysone , sphingidae , biology , chromatography , biochemistry , fast atom bombardment , hydrolysis , insect , mass spectrometry , chemistry , botany , mathematical analysis , mathematics , hormone , gene
The major ecdysteroid conjugate present in eggs (48–64 h old) of the tobacco hornworm has been purified by XAD‐2 chromatography, C 18 SEP‐PAK separations, and ion suppression reversed‐phase high‐performance liquid chromatography. Enzymatic hydrolysis of the conjugate with acid phosphatase from human seminal fluid gave 26‐hydroxyecdysone. The conjugate was identified as 26‐hydroxyecdysone 26‐phosphate by nuclear magnetic resonance and fast atom bombardment mass spectrometry. This compound is also the major conjugate of newly laid eggs (0–1 h old) of the tobacco hornworm. The role for ecdysteroid conjugates is discussed.