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Identification of 20‐hydroxyecdysone and ecdysone from the pupa of the gypsy moth, Lymantria dispar
Author(s) -
Feldlaufer M. F.,
Lusby W. R.,
Svoboda J. A.,
Thompson M. J.
Publication year - 1984
Publication title -
archives of insect biochemistry and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.576
H-Index - 66
eISSN - 1520-6327
pISSN - 0739-4462
DOI - 10.1002/arch.940010404
Subject(s) - lymantria dispar , gypsy moth , ecdysone , 20 hydroxyecdysone , pupa , biology , radioimmunoassay , dry weight , chromatography , lepidoptera genitalia , larva , botany , biochemistry , chemistry
Normal and reverse‐phase high‐performance liquid chromatography in conjunction with radioimmunoassay and mass spectrometry were used to identify the free and conjugated ecdysteroids (after enzymatic hydrolysis) from day‐4 pupae of the gypsy moth, Lymantria dispar L. Seven ecdysteroids were searched for, but only 20‐hydroxyecdysone (964 ng/g fresh weight) and ecdysone (367 ng/g fresh weight) were detected. Analysis of conjugated ecdysteriods after liberation by hydrolysis also indicated the presence of 20‐hydroxyecdysone (21.6 ng/g fresh weight) and ecdysone (2.4 ng/g fresh weight). Neither 26‐hydroxyecdysone nor the 3α‐epimers of 20‐hydroxyecdysone or ecdysone were detected.