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Expression of 20‐hydroxyecdysone‐related genes during gonadal development of Teleogryllus emma (Orthoptera: Gryllidae)
Author(s) -
Jin Wenjie,
Tan E.,
GharteyKwansah George,
Jia Yishu,
Xi Gengsi
Publication year - 2021
Publication title -
archives of insect biochemistry and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.576
H-Index - 66
eISSN - 1520-6327
pISSN - 0739-4462
DOI - 10.1002/arch.21824
Subject(s) - biology , 20 hydroxyecdysone , rna interference , gene , nymph , gene expression , insect , orthoptera , medicine , instar , endocrinology , development of the gonads , juvenile hormone , genetics , rna , botany , gonad , zoology , larva
Insect gonads develop under endocrine signals. In this study, we assessed the characters of partial complementary DNAs encoding the Teleogryllus emma orthologs of 20‐hydroxyecdysone (20E)‐related genes ( RXR, E75, HR3, Hsc70 , and Hsp90 ) and analyzed their expression patterns in both nymph and adult crickets. 20E treatment suppressed expression of TeEcR, TeRXR, TeE75, TeHR3, TeHsc70 , and TeHsp90 . Temporal expression analysis demonstrated that TeERR and 20E‐related genes were expressed in four stages of gonadal development from the fourth‐instar nymph stage to the adult stage. The expression pattern of these genes differed in testicular and ovarian development. TeRXR, HR3, TeHsc70 , and TeHsp90 were irregularly expressed in gonads of the same developmental stages, while mRNAs encoding TeERR, TeEcR , and TeE75 accumulated in higher levels in ovaries than in testes. RNA interference (RNAi) of TeEcR expression led to decrease of the expression levels of TeEcR, TeRXR, TeHR3 , and TeHsc70 , while it enhanced TeE75 and TeHsp90 expressions. These results demonstrate that the TeERR and 20E‐related genes help regulate gonadal development, while Te EcR appears to inhibit TeE75 expression, Te E75 inhibits HR3 expression. Hsc70 indirectly regulated the expression of the primary and secondary response genes E74A, E75B, and HR3. Hsp90 regulated Usp expression with no direct regulatory relationship with EcR .