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Apoptotic and autophagic characteristics of perivisceral fat body remodeling of the greater wax moth Galleria mellonella and effects of juvenile hormone analog, fenoxycarb, on these processes
Author(s) -
Poyraz Tinartas Esen,
Goncu Ebru,
Koc Kamil
Publication year - 2021
Publication title -
archives of insect biochemistry and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.576
H-Index - 66
eISSN - 1520-6327
pISSN - 0739-4462
DOI - 10.1002/arch.21780
Subject(s) - galleria mellonella , juvenile hormone , biology , autophagy , programmed cell death , metamorphosis , microbiology and biotechnology , ecdysone , apoptosis , 20 hydroxyecdysone , ecdysone receptor , hormone , medicine , endocrinology , larva , biochemistry , botany , nuclear receptor , virulence , transcription factor , gene
In holometabolous insects, many tissues and organs such as the fat body and midgut undergo a remodeling process during metamorphosis. Larval fat body cells are eliminated by programmed cell death (PCD), while tissue cells that adapt to adult life are formed by stem cells. In this study, we analyzed the features of the remodeling period of Galleria mellonella fat body in terms of PCD types, apoptotic and autophagic cell death characteristics. Besides, the effects of juvenile hormone (JH) on these processes were evaluated under the modified hormonal conditions via applications of JH analog, fenoxycarb. Several hallmarks of apoptotic and autophagic cell death were analyzed by morphological, biochemical, and molecular methods. The results of the present study have ascertained that the degeneration process of larval cells occurs via autophagic cell death accompanied by caspase‐3 activity during the pupal period and it is regulated by 20‐hydroxyecdysone (20HE) mediated by ecdysone receptor B1 (EcR‐B1). Increased activity of the acid phosphatase and upregulation of ATG6 and ATG8 in parallel with the formation of autophagosomes in the fat body of Galleria during the pupal period strongly indicated that autophagy was the key player in the remodeling processes.