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Isolation and expression of five genes in the mevalonate pathway of the Chinese white pine beetle, Dendroctonus armandi (Curculionidae: Scolytinae)
Author(s) -
Sun Yaya,
Dai Lulu,
Kang Xiaotong,
Fu Danyang,
Gao Haiming,
Chen Hui
Publication year - 2021
Publication title -
archives of insect biochemistry and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.576
H-Index - 66
eISSN - 1520-6327
pISSN - 0739-4462
DOI - 10.1002/arch.21760
Subject(s) - biology , curculionidae , mevalonate pathway , dendroctonus , white (mutation) , juvenile hormone , gene , botany , insect , bark beetle , genetics , biosynthesis
The Chinese white pine beetle Dendroctonus armandi (Tsai and Li) is a significant pest of the Qinling and Bashan Mountains pine forests of China. The Chinese white pine beetle can overcome the defences of Chinese white pine Pinus armandi (Franch) through pheromone‐assisted aggregation that results in a mass attack of host trees. We isolated five full‐length complementary DNAs encoding mevalonate pathway‐related enzyme genes from the Chinese white pine beetle ( D. armandi ), which are acetoacetyl‐CoA thiolase ( AACT ), geranylgeranyl diphosphate synthase ( GGPPS ), mevalonate kinase ( MK ), mevalonate diphosphate decarboxylase ( MPDC ), and phosphomevalonate kinase ( PMK ). Bioinformatic analyses were performed on the full‐length deduced amino acid sequences. Differential expression of these five genes was observed between sexes, and within these significant differences among topically applied juvenile hormone III (JH III), fed on phloem of P. armandi , tissue distribution, and development stage. Mevalonate pathway genes expression were induced by JH III and feeding.