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Insecticidal and synergistic activity of dsRNAs targeting buprofezin‐specific genes against the small brown planthopper, Laodelphax striatellus
Author(s) -
Fang Ying,
Park Min G.,
Choi Jae Y.,
Park Dong H.,
Wang Minghui,
Kim Hyun J.,
Kim Woo J.,
Je Yeon H.
Publication year - 2020
Publication title -
archives of insect biochemistry and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.576
H-Index - 66
eISSN - 1520-6327
pISSN - 0739-4462
DOI - 10.1002/arch.21739
Subject(s) - brown planthopper , rna interference , biology , gene , pest analysis , transcriptome , rna silencing , delphacidae , gene silencing , planthopper , genetics , rna , gene expression , botany , homoptera , hemiptera
The small brown planthopper, Laodelphax striatellus , is a dangerous pest in rice fields. Although buprofezin has been used to control L. striatellus for more than a decade, the occurrence of buprofezin‐resistant L. striatellus has been recently reported. To develop an alternative pest control strategy, comparative transcriptome analysis of buprofezin‐treated and nontreated L. striatellus was performed to screen the buprofezin‐specific target genes for RNA interference (RNAi) application. Among six genes downregulated in the buprofezin‐treated L. striatellus , RNAi‐based silencing of the lipophorin precursor, endocuticle structure glycoprotein, and chitin synthase significantly induced the lethality of L. striatellus in a concentration‐dependent manner. In addition, a cocktail of double‐stranded RNAs against these three genes showed synergistic effects with buprofezin. These results provide RNAi‐based effective approaches to control L. striatellus as well as an efficient method to identify novel target genes for RNAi application.