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Hesperidin modulates the rhythmic proteomic profiling in Drosophila melanogaster under oxidative stress
Author(s) -
Jayapalan Jaime J.,
Subramanian Perumal,
Kani Akshaya,
Hiji Jumriah,
Najjar Sara G.,
AbdulRahman Puteri S.,
Hashim Onn H.
Publication year - 2020
Publication title -
archives of insect biochemistry and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.576
H-Index - 66
eISSN - 1520-6327
pISSN - 0739-4462
DOI - 10.1002/arch.21738
Subject(s) - biology , rotenone , oxidative stress , proteome , hesperidin , drosophila melanogaster , proteomics , microbiology and biotechnology , hsp70 , biochemistry , heat shock protein , mitochondrion , medicine , alternative medicine , pathology , gene
The circadian clock regulates vital aspects of physiology including protein synthesis and oxidative stress response. In this investigation, we performed a proteome‐wide scrutiny of rhythmic protein accrual in Drosophila melanogaster on exposure to rotenone, rotenone + hesperidin and hesperidin in D. melanogaster . Total protein from fly samples collected at 6 h intervals over the 24 h period was subjected to two‐dimensional gel electrophoresis and mass spectrometry. Bioinformatics tool, Protein ANalysis THrough Evolutionary Relationships classification system was used to the determine the biological processes of the proteins of altered abundance. Conspicuous variations in the proteome (151 proteins) of the flies exposed to oxidative stress (by rotenone treatment) and after alleviating oxidative stress (by hesperidin treatment) were observed during the 24 h cycle. Significantly altered levels of abundance of a wide variety of proteins under oxidative stress (rotenone treatment) and under alleviation of oxidative stress (rotenone + hesperidin treatment) and hesperidin (alone) treatment were observed. These proteins are involved in metabolism, muscle activity, heat shock response, redox homeostasis, protein synthesis/folding/degradation, development, ion‐channel/cellular transport, and gustatory and olfactory function of the flies. Our data indicates that numerous cellular processes are involved in the temporal regulation of proteins and widespread modulations happen under rotenone treatment and, action of hesperidin could also be seen on these categories of proteins.