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CO 2 anesthesia on Drosophila survival in aging research
Author(s) -
Shen Jie,
Yang Peijing,
Zhu Xiang,
Gu Yitian,
Huang Jiahong,
Li Motao
Publication year - 2020
Publication title -
archives of insect biochemistry and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.576
H-Index - 66
eISSN - 1520-6327
pISSN - 0739-4462
DOI - 10.1002/arch.21639
Subject(s) - fecundity , drosophila melanogaster , drosophila (subgenus) , biology , oxidative stress , anesthesia , physiology , medicine , endocrinology , genetics , gene , population , environmental health
Carbon dioxide (CO 2 ) exposure is a common method of anesthesia in studies of Drosophila melanogaster . A number of negative side effects of CO 2 anesthesia have been reported. It is not clear whether the length of CO 2 anesthesia time affects Drosophila survival in aging research. Here, we examined the potential effect of the CO 2 anesthesia time length of 10–150 min. We found that long CO 2 exposure could lead to Drosophila death, more significant in males. The longer the anesthesia time is, the longer it takes for flies to wake up. Long‐time CO 2 anesthesia can reduce the lifespan. Our stress tests showed that long‐time CO 2 anesthesia can increase the average survival time in both males and females under starvation conditions, but can only increase female lifespan under H 2 O 2 oxidative stress. Long‐time CO 2 anesthesia also significantly affects physiological traits, with spontaneous activity increased in females but decreased in males, and reduced female fecundity. Our study suggests that limiting the CO 2 anesthesia time and giving enough recovery time before performing physiological tests are important in Drosophila aging research.