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Inorganic pyrophosphatase from the red flour beetle ( Tribolium castaneum ) modulates mitochondrial polyphosphate metabolism
Author(s) -
Mirra Bianca,
Carvalho Klébea,
Curitiba Bianca,
Ribeiro Lupis,
Moraes Jorge,
da Silva José R.,
Costa Evenilton P.,
da Fonseca Rodrigo N.,
Campos Eldo
Publication year - 2019
Publication title -
archives of insect biochemistry and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.576
H-Index - 66
eISSN - 1520-6327
pISSN - 0739-4462
DOI - 10.1002/arch.21606
Subject(s) - pyrophosphatase , polyphosphate , red flour beetle , biology , inorganic pyrophosphatase , mitochondrion , biochemistry , metabolism , atpase , microbiology and biotechnology , enzyme , insect , pyrophosphate , botany , phosphate
Polyphosphates (polyPs) have been found in all cell types examined to date and play diverse roles, depending on the cell type. In eukaryotic organisms, polyPs have been mainly investigated in mammalian cells, with few studies on insects. In this study, we investigated mitochondrial polyphosphate metabolism in the red flour beetle, Tribolium castaneum . Substrate specificity for different chain lengths demonstrated the presence of two exopolyphosphatase isoforms in mitochondria. T. castaneum mitochondrial polyP levels decreased after injection with soluble pyrophosphatase ( Tc‐sPPase ) dsRNA, while the membrane exopolyphosphate activity increased. Mitochondrial respiration modulated exopolyphosphatase activity only in wild‐type beetles. Tripolyphosphate was able to increase the F‐ATPase activity in wild‐type and Tc‐sPPase RNAi beetles. We suggest that inorganic pyrophosphatase modulates polyphosphate metabolism in mitochondria and affects the link between mitochondrial activity and polyphosphate metabolism in T. castaneum .