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Effect of natural toxins and adipokinetic hormones on the activity of digestive enzymes in the midgut of the cockroach Periplaneta americana
Author(s) -
Karbusová Nikola,
Gautam Umesh K.,
Kodrík Dalibor
Publication year - 2019
Publication title -
archives of insect biochemistry and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.576
H-Index - 66
eISSN - 1520-6327
pISSN - 0739-4462
DOI - 10.1002/arch.21586
Subject(s) - periplaneta , midgut , cockroach , biology , adipokinetic hormone , metarhizium anisopliae , dictyoptera , digestive enzyme , entomopathogenic fungus , enzyme , metarhizium , microbiology and biotechnology , hemolymph , biochemistry , botany , larva , biological pest control , ecology , amylase , fat body , beauveria bassiana
This study examined the effect of two natural toxins (a venom from the parasitic wasp Habrobracon hebetor and destruxin A from the entomopathogenic fungus Metarhizium anisopliae ), and one pathogen (the entomopathogenic fungus Isaria fumosorosea ) on the activity of basic digestive enzymes in the midgut of the cockroach Periplaneta americana . Simultaneously, the role of adipokinetic hormones (AKH) in the digestive processes was evaluated. The results showed that all tested toxins/pathogens elicited stress responses when applied into the cockroach body, as documented by an increase of AKH level in the central nervous system. The venom from H. hebetor showed no effect on digestive enzyme activities in the ceca and midgut in vitro. In addition, infection by I. fumosorosea caused a decrease in activity of all enzymes in the midgut and a variable decrease in activity in the ceca; application of AKHs did not reverse the inhibition. Destruxin A inhibited the activity of all enzymes in the midgut but none in the ceca in vitro; application of AKHs did reverse this inhibition, and no differences between both cockroach AKHs were found. Overall, the results demonstrated the variable effect of the tested toxins/pathogens on the digestive processes of cockroaches as well as the variable ability of AKH to counteract these effects.