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Molecular characterization and expression patterns of Phenacoccus solenopsis (Hemiptera: Pseudococcidae) heat shock protein genes and their response to host stress
Author(s) -
Liu Dan,
Dang Xiangli,
Song Wei,
Xi Lingyu,
Wang Qi,
Zhang Shaobing,
Miao Yong,
Li Guiting,
Jiang Junqi
Publication year - 2019
Publication title -
archives of insect biochemistry and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.576
H-Index - 66
eISSN - 1520-6327
pISSN - 0739-4462
DOI - 10.1002/arch.21536
Subject(s) - biology , heat shock protein , nymph , gene , hemiptera , transcriptome , host (biology) , gene expression , microbiology and biotechnology , downregulation and upregulation , botany , genetics
As a polyphagous insect, little is known at the molecular level about the effects of different host plants on physiological changes in Phenacoccus solenopsis . In this study, four heat shock protein (Hsp) genes ( PsHsp60 , PsHsp70 , PsHsp90 , and PsHsp20.7 ) were identified from the transcriptome of P. solenopsis . Analysis of Hsp expression levels revealed significant differences in Hsp gene expression levels in P. solenopsis fed on different host plants. In host conversion tests, the expression levels of PsHsp90 and PsHsp60 were upregulated after transfer of second instar nymphs from tomato to cotton. The expression levels of PsHsp70 and PsHsp20.7 were, respectively, significantly upregulated at 9 and 48 hr after transfer from tomato to Hibiscus . The results of this study aid molecular characterization and understanding of the expression patterns of Hsp genes during different developmental stages and host transfer of P. solenopsis .