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Pheromone‐binding proteins in the Asian gypsy moth females, Lymantria dispar , recognizing the sex pheromone and plant volatiles
Author(s) -
Yu Yanxue,
Zhou Ping,
Zhang Junhua,
Zheng Chao,
Zhang Jian,
Chen Naizhong
Publication year - 2018
Publication title -
archives of insect biochemistry and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.576
H-Index - 66
eISSN - 1520-6327
pISSN - 0739-4462
DOI - 10.1002/arch.21477
Subject(s) - biology , gypsy moth , lymantria dispar , pheromone , rna interference , gene , botany , genetics , rna , pest analysis , lepidoptera genitalia
Lepidopterans are known to have different pheromone‐binding proteins with differential expression patterns that facilitate specific signal transduction of semiochemicals. Two PBPs of the Asian gypsy moth, Lymantria dispar , were reported to express in both females and males, but their physiological functions were unknown. Results showed that Ldis PBP1 and Ldis PBP2 were expressed in the sensilla trichodea of males and the s. trichodea and s. basiconica of females. When Ldis PBP1 gene was targeted by RNA interference (RNAi) in males, the expression of Ldis PBP1 and Ldis PBP2 decreased by 69 and 76%, respectively, and when Ldis PBP2 gene was targeted by RNAi, they decreased by 60 and 42%, respectively. In females, after treatment with Ldis PBP1 dsRNA, Ldis PBP1 and Ldis PBP2 levels were reduced by 26 and 69%, respectively, and Ldis PBP2 dsRNA reduced the relative expression of them by 4 and 62%, respectively. The expression of Ldis PBP1 and Ldis PBP2 was interdependent. Electroantennogram (EAG) recordings showed that Ldis PBPs participate in the recognition of the sex pheromone in males, and the sex pheromone and plant volatiles in females. The function of Ldis PBPs represents the sex‐specific roles.