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Carbonic anhydrase II confers resistance to deltamethrin in Culex pipiens pallens
Author(s) -
Ye Wenyun,
Zhang Cheng,
Xu Na,
Sun Yan,
Ma Lei,
Shen Bo,
Zhou Dan,
Zhu Changliang
Publication year - 2017
Publication title -
archives of insect biochemistry and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.576
H-Index - 66
eISSN - 1520-6327
pISSN - 0739-4462
DOI - 10.1002/arch.21428
Subject(s) - deltamethrin , biology , enzyme , carbonic anhydrase , culex pipiens , biochemistry , gene , strain (injury) , genetics , botany , pesticide , ecology , larva , anatomy
Carbonic anhydrases (CAs) are metabolic enzymes that regulate the physiological equilibrium in a variety of organisms. In this study, we investigated the function of CA II in Culex pipiens pallens using real‐time quantitative polymerase chain reaction and double‐stranded RNA injection. CA II transcripts were more abundant in the deltamethrin‐susceptible strain than the deltamethrin‐resistant strain. The activities of metabolic enzymes increased when the CA II expression was silenced. These findings suggest CA II regulates deltamethrin resistance by altering metabolic enzyme activity, and could serve as a potential genetic marker for monitoring deltamethrin resistance in mosquitoes.