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Dietary TiO 2 particles modulate expression of hormone‐related genes in Bombyx mori
Author(s) -
Shi Guofang,
Zhan Pengfei,
Jin Weiming,
Fei JianMing,
Zhao Lihua
Publication year - 2017
Publication title -
archives of insect biochemistry and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.576
H-Index - 66
eISSN - 1520-6327
pISSN - 0739-4462
DOI - 10.1002/arch.21397
Subject(s) - biology , bombyx mori , pi3k/akt/mtor pathway , ecdysteroid , endocrinology , medicine , signal transduction , insulin receptor , downregulation and upregulation , hormone receptor , insulin , protein kinase b , hormone , microbiology and biotechnology , gene , biochemistry , insulin resistance , genetics , cancer , breast cancer
Silkworm ( Bombyx mori ) is an economically beneficial insect. Its growth and development are regulated by endogenous hormones. In the present study, we found that feeding titanium dioxide nanoparticles (TiO 2 NP) caused a significant increase of body size. TiO 2 NP stimulated the transcription of several genes, including the insulin‐related hormone bombyxin, PI3K/Akt/TOR (where PI3K is phosphatidylinositol 3‐kinase and TOR is target of rapamycin), and the adenosine 5′‐monophosphateactivated protein kinase (AMPK)/target of rapamycin (TOR) pathways. Differentially expressed gene (DEG) analysis documented 26 developmental hormone signaling related genes that were differentially expressed following dietary TiO 2 NP treatment. qPCR analysis confirmed the upregulation of insulin/ecdysteroid signaling genes, such as bombyxin B‐1 , bombyxin B‐4 , bombyxin B‐7 , MAPK , P70S6K , PI3k , eIF4E , E75 , ecdysteroid receptor ( EcR ), and insulin‐related peptide binding protein precursor 2 ( IBP2 ). We infer from the upregulated expression of bombyxins and the signaling network that they act in bombyxin ‐stimulated ecdysteroidogenesis.