z-logo
Premium
Dietary TiO 2 particles modulate expression of hormone‐related genes in Bombyx mori
Author(s) -
Shi Guofang,
Zhan Pengfei,
Jin Weiming,
Fei JianMing,
Zhao Lihua
Publication year - 2017
Publication title -
archives of insect biochemistry and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.576
H-Index - 66
eISSN - 1520-6327
pISSN - 0739-4462
DOI - 10.1002/arch.21397
Subject(s) - biology , bombyx mori , pi3k/akt/mtor pathway , ecdysteroid , endocrinology , medicine , signal transduction , insulin receptor , downregulation and upregulation , hormone receptor , insulin , protein kinase b , hormone , microbiology and biotechnology , gene , biochemistry , insulin resistance , genetics , cancer , breast cancer
Silkworm ( Bombyx mori ) is an economically beneficial insect. Its growth and development are regulated by endogenous hormones. In the present study, we found that feeding titanium dioxide nanoparticles (TiO 2 NP) caused a significant increase of body size. TiO 2 NP stimulated the transcription of several genes, including the insulin‐related hormone bombyxin, PI3K/Akt/TOR (where PI3K is phosphatidylinositol 3‐kinase and TOR is target of rapamycin), and the adenosine 5′‐monophosphateactivated protein kinase (AMPK)/target of rapamycin (TOR) pathways. Differentially expressed gene (DEG) analysis documented 26 developmental hormone signaling related genes that were differentially expressed following dietary TiO 2 NP treatment. qPCR analysis confirmed the upregulation of insulin/ecdysteroid signaling genes, such as bombyxin B‐1 , bombyxin B‐4 , bombyxin B‐7 , MAPK , P70S6K , PI3k , eIF4E , E75 , ecdysteroid receptor ( EcR ), and insulin‐related peptide binding protein precursor 2 ( IBP2 ). We infer from the upregulated expression of bombyxins and the signaling network that they act in bombyxin ‐stimulated ecdysteroidogenesis.

This content is not available in your region!

Continue researching here.

Having issues? You can contact us here