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Identification of β‐chain of F o F 1 ‐ATPase in apoptotic cell population induced by Microplitis bicoloratus bracovirus and its role in the development of Spodoptera litura
Author(s) -
Kou TianChao,
Liu YueTong,
Li Ming,
Yang Yang,
Zhang Wei,
Cui JiHui,
Zhang XueWen,
Dong ShuMei,
Xu Sha,
You Shan,
Yu DongShuai,
Pang ZunYu,
Luo KaiJun
Publication year - 2017
Publication title -
archives of insect biochemistry and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.576
H-Index - 66
eISSN - 1520-6327
pISSN - 0739-4462
DOI - 10.1002/arch.21389
Subject(s) - biology , spodoptera litura , ectopic expression , apoptosis , atpase , rna interference , microbiology and biotechnology , population , rna , gene , biochemistry , botany , larva , enzyme , demography , sociology
Two physiological changes of Spodoptera litura parasitized by Microplitis bicoloratus are hemocyte‐apoptosis and retarded immature development. β‐Chain of F o F 1 ‐ATPase was found from a S. litura transcriptome. It belongs to a conserved P‐loop NTPase superfamily, descending from a common ancestor of Lepidopteran clade. However, the characterization of β‐chain of ATPase in apoptotic cells and its involvement in development remain unknown. Here, the ectopic expression and endogenous F o F 1 ‐ATPase β‐chain occurred on S. litura cell membrane: in vivo , at the late stage of apoptotic hemocyte, endogenous F o F 1 ‐ATPase β‐chain was stably expressed during M. bicoloratus larva development from 4 to 7 days post‐parasitization; in vitro , at an early stage of pre‐apoptotic Spli221 cells by infecting with M. bicoloratus bracovirus particles, the proteins were speedily recover expression. Furthermore, endogenous F o F 1 ‐ATPase β‐chain was localized on the apoptotic cell membrane. RNA interference (RNAi) of F o F 1 ‐ATPase β‐chain led to significantly decreased head capsule width. This suggested that F o F 1 ‐ATPase β‐chain positively regulated the development of S. litura . The RNAi effect on the head capsule width was enhanced with parasitism. Our research found that F o F 1 ‐ATPase β‐chain was expressed and localized on the cell membrane in the apoptotic cells, and involved in the development of S. litura .