Premium
VENOM FROM THE ECTOPARASITIC WASP Habrobracon hebetor ACTIVATES CALCIUM‐DEPENDENT DEGRADATION OF Galleria mellonella LARVAL HEMOCYTES
Author(s) -
Kryukova Natalia A.,
Chertkova Ekaterina A.,
Semenova Alexandra D,
Glazachev Yuri I.,
Slepneva Irina A.,
Glupov Victor V.
Publication year - 2015
Publication title -
archives of insect biochemistry and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.576
H-Index - 66
eISSN - 1520-6327
pISSN - 0739-4462
DOI - 10.1002/arch.21247
Subject(s) - galleria mellonella , biology , envenomation , hemolymph , venom , hemocyte , microbiology and biotechnology , parasitoid , larva , toxicology , immune system , zoology , immunology , virulence , ecology , biochemistry , gene
Ectoparasitoids inject venom into hemolymph during oviposition. We determined the influence of envenomation by the parasitoid, Habrobracon hebetor , on the hemocytes of its larval host, Galleria mellonella . An increase in both intracellular Са 2+ content and phospholipase C activity of the host hemocytes was recorded during 2 days following envenomation by the parasitoid. The decreased hemocyte viability was detected 1, 2, and 24 h after the envenomation. Injecting of the crude venom (final protein concentration 3 μg/ml) into the G. mellonella larvae led to the reduced hemocyte adhesion. The larval envenomation caused a decrease in transmembrane potential of the hemocytes. These findings document the suppression of hemocytic immune effectors in the parasitized host larvae.