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Ca 2+ /CALMODULIN‐DEPENDENT PROTEIN KINASE II IN THE COCKROACH Periplaneta americana : IDENTIFICATION OF FIVE ISOFORMS AND THEIR TISSUES DISTRIBUTION
Author(s) -
Taillebois Emiliane,
Heuland Emilie,
Bourdin Céline M.,
Griveau Audrey,
Quinchard Sophie,
TricoireLeignel Helene,
Legros Christian,
Thany Steeve H.
Publication year - 2013
Publication title -
archives of insect biochemistry and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.576
H-Index - 66
eISSN - 1520-6327
pISSN - 0739-4462
DOI - 10.1002/arch.21102
Subject(s) - biology , gene isoform , microbiology and biotechnology , alternative splicing , gryllus bimaculatus , periplaneta , american cockroach , isozyme , drosophila melanogaster , cockroach , biochemistry , gene , enzyme , ecology , cricket
Calcium/calmodulin‐dependent protein kinase II ( C a MKII ) is a key kinase that transduces C a 2+ signals into downstream effects acting on a range of cellular processes in nervous system and muscular tissues. In insects, different C a MKII isoforms have been reported in D rosophila melanogaster, A pis florae, B ombus terrestris, and B ombus impatiens but little is known on the organization and tissue‐specific expression of these isoforms with the exception of D rosophila. The present study reports the cloning of five C a MKII splice variants issued from a single gene and their tissue‐specific expression in the cockroach P eriplaneta americana. Each C a MKII isoform shared 82–90% identity with D rosophila C a MKII isoforms and accordingly were named P a C a MKII ‐ A , P a C a MKII ‐ B , P a C a MKII ‐ C , P a C a MKII ‐ D , and P a C a MKII ‐ E . P a C a MKII ‐ A and P a C a MKII ‐ D isoforms are ubiquitously expressed in all tissues, but some such as P a C a MKII ‐ B and P a C a MKII ‐ C are preferentially expressed in the nerve cord and muscle. In addition, using single‐cell reverse transcriptase‐polymerase chain reaction ( RT ‐ PCR ), we found a tissue‐specific expression of P a C a MKII ‐ E in the dorsal unpaired median neurons. Alternative splicing of P a C a MKII transcripts is likely a common mechanism in insects to control the pattern of isoform expression in the different tissues.