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EFFECTS OF GENETIC KNOCK‐DOWN OF ORGANIC ANION TRANSPORTER GENES ON SECRETION OF FLUORESCENT ORGANIC IONS BY MALPIGHIAN TUBULES OF D rosophila melanogaster
Author(s) -
Chahine Sarah,
Seabrooke Sara,
O'Donnell Michael J.
Publication year - 2012
Publication title -
archives of insect biochemistry and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.576
H-Index - 66
eISSN - 1520-6327
pISSN - 0739-4462
DOI - 10.1002/arch.21066
Subject(s) - secretion , transporter , biology , efflux , p glycoprotein , organic anion transporting polypeptide , organic anion transporter 1 , gene expression , biochemistry , microbiology and biotechnology , atp binding cassette transporter , fluorescence , green fluorescent protein , gene , multiple drug resistance , antibiotics , physics , quantum mechanics
An earlier study has shown that RNA i knock‐down of a single organic anion transporter ( OAT ) gene in the principal cells of D rosophila M alpighian tubules is associated with reductions in the expression of multiple, functionally related genes. In this study, we measured the rates of secretion of four fluorescent ions by tubules isolated from flies expressing targeted RNA i knock‐down of specific OAT genes. Droplets secreted by isolated tubules set up in the Ramsay assay were collected in optically flat capillary tubes and the concentrations of fluorescent ions were determined by confocal laser scanning microscopy. Reductions in the expression of organic anion (OA) transporting polypeptide 58 D c ( OATP ; CG 3380) were associated with reduced secretion of the OA s fluorescein and T exas R ed . Reduction in the expression of Drosophila multidrug resistance associated protein (d MRP ; CG 6214) was correlated with reduced secretion of the P ‐glycoprotein substrate daunorubicin. Secretion of the organic cation quinacrine was unaffected by reduced expression of OATP , d MRP , or a multidrug efflux transporter ( MET ; CG 30344). The results highlight the difficulties of assigning a rate‐limiting role in transport of a specific OA to a single membrane transporter .