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GENETIC TRANSFORMATION MEDIATED BY piggyBac IN THE A SIAN CORN BORER, O strinia furnacalis ( L EPIDOPTERA: C RAMBIDAE)
Author(s) -
Liu Dan,
Yan Shanchun,
Liu Dan,
Huang Yongping,
Tan Anjiang,
Stanley David W.,
Song Qisheng
Publication year - 2012
Publication title -
archives of insect biochemistry and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.576
H-Index - 66
eISSN - 1520-6327
pISSN - 0739-4462
DOI - 10.1002/arch.21035
Subject(s) - ostrinia furnacalis , biology , transgene , transformation (genetics) , microbiology and biotechnology , genetically modified maize , crambidae , gene , green fluorescent protein , lepidoptera genitalia , expression vector , southern blot , microinjection , genetically modified crops , genetics , botany , recombinant dna
The Asian corn borer, O strinia furnacalis , is a serious pest of corn, sorghum, and cotton in C hina and other A sian countries. The present study is the first attempt to establish the transgenic line in O . furnacalis using a piggy B ac transposon, which will shed light on the future genetic control of O . furnacalis . A piggy B ac vector p B ac[ A 3 EGFP ] was constructed to express enhanced green fluorescence protein ( EGFP) under the control of Bombyx mori actin3 promoter. Transient EGFP expression was detected 48 h after preblastodermic microinjection of p B ac[ A 3 EGFP ] and the excision assay showed the transgenic vector was precisely excised. In G 1 animals, PCR (polymerase chain reaction)‐based investigations revealed that the exogenous gene had been introduced into O . furnacalis genome and expressed at the transcriptional level. W estern blot analysis showed EGFP expression at the protein level, indicating the heritability of the transgene.