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SEQUENCING AND CHARACTERIZATION OF GLYCOGEN SYNTHASE AND GLYCOGEN PHOSPHORYLASE GENES FROM S podoptera exigua AND ANALYSIS OF THEIR FUNCTION IN STARVATION AND EXCESSIVE SUGAR INTAKE
Author(s) -
Tang Bin,
Xu Qi,
Zou Qi,
Fang Qi,
Wang Shigui,
Ye Gongyin
Publication year - 2012
Publication title -
archives of insect biochemistry and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.576
H-Index - 66
eISSN - 1520-6327
pISSN - 0739-4462
DOI - 10.1002/arch.21027
Subject(s) - biology , glycogen , glycogen phosphorylase , open reading frame , trehalose , biochemistry , glycogen synthase , exigua , rna , gene , microbiology and biotechnology , peptide sequence , spodoptera , recombinant dna
Glycogen and trehalose are important energy source and key regulation factors in the development of many organisms’ pass through energy metabolism, including bacteria, fungi, and insects. To study glycogen metabolism pathway in S podoptera exigua, first c DNA s for glycogen synthase ( S poex GS ) and glycogen phosphorylase ( S poex GP ) were cloned from S . exigua. S poex GS c DNA contains an open reading frame of 2,010 nucleotides encoding a protein of 669 amino acids with a predicted molecular mass of 76.19 kDa and a pI of 5.84. S poex GP contains an open reading frame of 2,946 nucleotides, which encodes a protein of 841 amino acids with a predicted molecular mass of approximately 96.63 kDa and a p I of 6.03. Second, N orthern blotting revealed that S poex GS and S poex GP m RNA s were expressed in brain, fat body, mid‐gut, M alpighian tubules, spermary, and tracheae of S . exigua. Expression patterns for S poex GS and S poex GP m RNA s were similar in fat body, but differed in whole body at different developmental stages. The last, under starvation conditions, S poex GS and S poex GP transcript expression rapidly decreased with increasing starvation time. When the starvation stress was removed, S poex GS and S poex GP m RNA levels were lower in the groups starved for 6 and 12 h than in the 24‐h starvation and control groups. Treatment with excessive sugar intake led to higher levels of S poex GS and S poex GP transcripts after 12 h compared to the control group. These findings provide new data on the tissue distribution, expression patterns, and potential function of glycogen synthase and glycogen phosphorylase proteins. © 2012 Wiley Periodicals, Inc.