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EFFECTS OF THE MICROBIAL METABOLITE DESTRUXIN A ON ION TRANSPORT BY THE GUT AND RENAL EPITHELIA OF Drosophila melanogaster
Author(s) -
RuizSanchez Esau,
O'Donnell Michael J.
Publication year - 2012
Publication title -
archives of insect biochemistry and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.576
H-Index - 66
eISSN - 1520-6327
pISSN - 0739-4462
DOI - 10.1002/arch.21023
Subject(s) - biology , hemolymph , metabolite , insect , midgut , drosophila melanogaster , metabolism , malpighian tubule system , toxin , biochemistry , botany , larva , gene
Destruxins have been implicated in the infection process by entomopathogenic fungi and have been also found to be highly toxic when applied topically or ingested by different insect species. To gain insight into the mechanism of action of this toxin on insect internal organs, we have evaluated the effects of destruxin A on D rosophila melanogaster M alpighian tubules and gut tissues. Destruxin A was toxic when injected into adults; the calculated EC 50 was 0.11 m M . Destruxin A significantly inhibited fluid secretion rate by M alpighian tubules as well; the calculated IC 50 was 0.25 μ M . The N a + concentration in the secreted fluid increased significantly when tubules were exposed to 0.25 μ M destruxin A , whereas p H and the concentrations of C a 2+ and K + did not change. In gut, there was no effect of destruxin on H + flux, but there was a significant decrease in K + and C a 2+ absorption. The concentration of C a 2+ and K + in the hemolymph of destruxin A ‐injected flies was not significantly different from those of control flies after 3 h. Taken together, these results show that destruxin A produces differential effects on ion transport by renal and gut tissues. © 2012 Wiley Periodicals, Inc.