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THE INVOLVEMENT OF CYTOCHROME P450 MONOOXYGENASES IN METHANOL ELIMINATION IN Drosophila melanogaster LARVAE
Author(s) -
Wang ShuPing,
He GuiLing,
Chen RuiRui,
Li Fei,
Li GuoQing
Publication year - 2012
Publication title -
archives of insect biochemistry and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.576
H-Index - 66
eISSN - 1520-6327
pISSN - 0739-4462
DOI - 10.1002/arch.21021
Subject(s) - monooxygenase , piperonyl butoxide , methanol , cytochrome p450 , drosophila melanogaster , biology , biochemistry , metabolism , xenobiotic , enzyme , chemistry , gene , organic chemistry , pesticide , agronomy
Methanol is one of the most common short‐chain alcohols in fermenting fruits, the natural food of the fruit fly, D rosophila melanogaster. The larvae cope continuously with methanol at various concentrations in order to survive and develop. In the present article, we found toxicities of dietary methanol and formaldehyde were enhanced by piperonyl butoxide, but not by 3‐amino‐1, 2, 4‐triazole, 4‐methylpyrazole, diethylmeleate, and triphenyl phosphate, when assessing by the combination index method. These results reveal that cytochrome P 450 monooxygenases ( CYP s), rather than catalases, alcohol dehydrogenases, glutathione S ‐transferases, and esterases, participate in methanol metabolism. Moreover, methanol exposure dramatically increased CYP activity. The ratios of the CYP activities in treated larvae to those in control reached, respectively, up to 3.0‐, 3.9‐, and 2.7‐fold, at methanol concentrations of 22.6, 27.9, and 34.5 mg/g diet. In addition, methanol exposure greatly up‐regulated the m RNA expression level of five C yp genes, which were C yp304a1, C yp9f2, C yp28a5, C yp4d2, and C yp4e2. Their resulting proteins were suggested as the candidate enzymes for methanol metabolism in D . melanogaster larvae. © 2012 Wiley Periodicals, Inc.