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RNA INTERFERENCE OF TWO ACETYLCHOLINESTERASE GENES IN P lutella xylostella REVEALS THEIR DIFFERENT FUNCTIONS
Author(s) -
He Guiling,
Sun Yang,
Li Fei
Publication year - 2012
Publication title -
archives of insect biochemistry and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.576
H-Index - 66
eISSN - 1520-6327
pISSN - 0739-4462
DOI - 10.1002/arch.21007
Subject(s) - acetylcholinesterase , biology , rna interference , plutella , rna , instar , gene knockdown , gene , aché , larva , gene silencing , acetylcholine , carboxylesterase , enzyme , messenger rna , rna silencing , microbiology and biotechnology , biochemistry , endocrinology , botany
Acetylcholinesterase ( AC hE, EC 3.1.1.7) is an important enzyme with a typical function of degrading the neurotransmitter acetylcholine. Although two ace genes were reported in P lutella xylostella, their function differences remain largely unknown. The chemically synthesized si RNA s (si‐ P xace1 and si‐ P xace2) were injected into the second instar larvae to knock down P xace1 and P xace2, either respectively or simultaneously. The m RNA abundance of P xace1 and P xace2 was significantly reduced to 7–33.5% of the control levels at 72 h after si RNA injection. The AC h E activities were significantly decreased at 96 h after treatment. Silencing of P xace1 or P xace2 resulted in mortality of 33.9 and 22.9%, respectively. The survivors in si RNA ‐treated groups had apparent growth inhibition such as reduction in larvae weights and lengths, malformation and motor retardation. Knockdown of P xace1 apparently affected more on larvae growth than that of P xace2, suggesting that P xace1 had more important roles than P xace2. Both P xace1 and P xace2 genes might have atypical functions in regulating larvae growth and motor ability.