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Microarray expression profiling of Spodoptera litura in response to oxidative stress
Author(s) -
Yun EunYoung,
Hwang JaeSam,
Yoon YoungIl,
Ahn MiYoung,
Kim NamJung,
Kwon OYu,
Lee WonJae,
Goo TaeWon
Publication year - 2011
Publication title -
archives of insect biochemistry and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.576
H-Index - 66
eISSN - 1520-6327
pISSN - 0739-4462
DOI - 10.1002/arch.20431
Subject(s) - spodoptera litura , biology , expressed sequence tag , gene , microarray , gene expression profiling , gene expression , microarray analysis techniques , complementary dna , microbiology and biotechnology , genetics , botany , larva
To examine the expression profile of oxidative stress responsive genes in Spodoptera litura , we constructed a cDNA library from S. litura injected with hydrogen peroxide (H 2 O 2 ). Using a microarray chip composed of 2,964 cDNAs, we screened gene expression at 1, 3, 5, 7, and 9 h post H 2 O 2 injection. Data were clustered into 15 groups of genes that behave similarly across each time course. Seventy‐three genes were identified as being at least twofold up‐ or downregulated after treatment with H 2 O 2 in S. litura . We constructed expressed sequence tags (ESTs) for genes that changed at least twofold after treatment with H 2 O 2 . The functional classification of these ESTs based on Gene Ontology showed that the ESTs are rich in genes involved in oxidoreductase activity (5.7%), defense (14.3%), cellular process (22.9%), and development (17.1%). © 2011 Wiley Periodicals, Inc.