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An epidermis‐specific chitin synthase CDNA in Choristoneura fumiferana : cloning, characterization, developmental and hormonal‐regulated expression
Author(s) -
Ampasala Dinakar R.,
Zheng Sichun,
Zhang Dayu,
Ladd, Tim,
Doucet Daniel,
Krell Peter J.,
Retnakaran Arthur,
Feng Qili
Publication year - 2011
Publication title -
archives of insect biochemistry and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.576
H-Index - 66
eISSN - 1520-6327
pISSN - 0739-4462
DOI - 10.1002/arch.20404
Subject(s) - biology , complementary dna , cloning (programming) , chitin synthase , molecular cloning , choristoneura fumiferana , epidermis (zoology) , chitin , hormone , microbiology and biotechnology , biochemistry , botany , spruce budworm , gene , tortricidae , larva , anatomy , chitosan , computer science , programming language
Chitin synthase catalyzes chitin synthesis in the exoskeleton, tracheal system and gut during insect development. A chitin synthase 1 ( Cf CHS1) cDNA was identified and cloned from the spruce budworm, Choristoneura fumiferana . The Cf CHS1 cDNA is 5,300 bp in length and codes a 1,564‐amino acid protein with a molecular mass of 178 kDa. The deduced protein contains 16 transmembrane helixes in its domains A and C. The single copy Cf CHS1 gene expressed during each of the larval molts from the 3rd to the 6th instar. The gene expressed highly and periodically in the epidermis during each of molts, whereas no transcripts were detected in the midgut and fat body. 20‐hydroxyecdysone and the ecdysone agonist RH5992 suppressed Cf CHS1 expression, whereas the juvenile hormone analog methoprene induced Cf CHS1 expression. These results implicate that Cf CHS1 is involved in the chitin synthase and new chitin formation during molting in the insect. © 2010 Wiley Periodicals, Inc.