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Putative‐farnesoic acid O ‐methyltransferase (FAMeT) in medfly reproduction
Author(s) -
Vannini Laura,
Ciolfi Silvia,
Dallai Romano,
Frati Francesco,
Hoffmann Klaus H.,
MeyeringVos Martina
Publication year - 2010
Publication title -
archives of insect biochemistry and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.576
H-Index - 66
eISSN - 1520-6327
pISSN - 0739-4462
DOI - 10.1002/arch.20382
Subject(s) - ceratitis capitata , biology , juvenile hormone , gene , gene silencing , rna interference , genetics , capitata , reproduction , mating , gene expression , methyltransferase , drosophila melanogaster , insect , zoology , rna , tephritidae , botany , pest analysis , methylation , brassica oleracea
A gene potentially involved in juvenile hormone (JH) biosynthesis was previously identified in Ceratitis capitata as the putative‐ farnesoic acid O‐methyltransferase (FAMeT). Since JH is involved in insect reproduction, we silenced the putative‐FAMeT expression by RNA interference in Ceratitis capitata to evaluate its implication in egg production. FAMeT gene expression was knocked down in females and males after eclosion and in 1‐ and 2‐day‐old females. Treated specimens were left to mate with each other or with untreated partners to evaluate the extent of each sex influencing egg production. Gene silencing was investigated by Real‐Time PCR. Results unambiguously showed that FAMeT has a measurable role on the fertility of both medfly sexes. © 2010 Wiley Periodicals, Inc.