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Molecular characterization of two nicotinic acetylcholine receptor subunits from Liposcelis bostrychophila Badonnel (Psocoptera: Liposcelididae)
Author(s) -
Tang PeiAn,
Jiang HongBo,
Xu YongQiang,
An FengMing,
Wang JinJun
Publication year - 2009
Publication title -
archives of insect biochemistry and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.576
H-Index - 66
eISSN - 1520-6327
pISSN - 0739-4462
DOI - 10.1002/arch.20321
Subject(s) - biology , psocoptera , open reading frame , orfs , nicotinic acetylcholine receptor , anopheles gambiae , genetics , amino acid , complementary dna , microbiology and biotechnology , gene , peptide sequence , nicotinic agonist , botany , receptor , malaria , immunology
Two nicotinic acetylcholine receptor (nAChR) subunit genes, Lb α1 and Lb α8, were isolated and characterized from psocid, Liposcelis bostrychophila Badonnel, using the rapid amplification of cDNA ends (RACE) technique. They are the first two nAChR family members isolated from the insect order of Psocoptera. The full‐length cDNAs of Lb α1 (GenBank accession number: EU871527) and Lb α8 (EU871526) consist of 2,025 and 1,763 nucleotides, respectively, and an open reading frame of 1,644 and 1,608 bp encoding 547 and 535 amino acid proteins, respectively. Both genes have typical features of nAChR family members, though they share only 56% identity in amino acid sequence. The dendrogram generated by the MEGA 3.1 program shows that the protein deduced by Lb α1 had the closest phylogenetic relationship to Agam α1 from Anopheles gambiae and Amel α1 from Apis mellifera , and Lb α8 shares the highest identity with Agam α8 from An. gambiae and Amel α8 from A. mellifera . Quantitative real‐time PCR analysis showed that Lb α1 was expressed 2.03–6.54‐fold higher than Lb α8 at the different developmental stages of L . bostrychophila . The highest expression levels of Lb α1 and Lb α8 were both detected at adult stage and the lowest were at the third and fourth nymphal stages, respectively. There was a stable and relatively low expression level for Lb α1, whereas there was a descending expression pattern for Lb α8 in the 1st through the 4th nymphal stadia. © 2009 Wiley Periodicals Inc.