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Juvenile hormone induction of glutathione S ‐transferase activity in the larval fat body of the common cutworm, Spodoptera litura (Lepidoptera: Noctuidae)
Author(s) -
Wu MingCheng,
Lu KuangHui
Publication year - 2008
Publication title -
archives of insect biochemistry and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.576
H-Index - 66
eISSN - 1520-6327
pISSN - 0739-4462
DOI - 10.1002/arch.20257
Subject(s) - biology , cutworm , spodoptera litura , noctuidae , juvenile hormone , instar , larva , lepidoptera genitalia , botany , cycloheximide , microbiology and biotechnology , protein biosynthesis
The effect of pyriproxyfen, a juvenile hormone analog (JHA), on the pupation of S. litura was examined. A topical application of 100 μg JHA/larva on the newly ecdysed (0‐day) sixth instar larvae resulted in more than 80% pupation, while most of the 1‐ or 2‐day‐old larvae similarly treated developed into supernumerary larvae. Glutathione S ‐transferse (GST) activity in the fat body of 0‐day‐old sixth instar larvae was significantly induced within 12 h of JHA (100 μg/larva) treatment. In contrast, no such induction was found when 1‐ and 2‐day‐old sixth instar larvae were similarly treated. This induction phenomenon was also observed when 0‐day‐old sixth instar larvae were treated with the natural JH III. The suppressive effects of α‐amanitin and cycloheximide suggest that JHA induction of GST activity in these cutworm larvae presumably occurred at the gene transcription level. Arch. Insect Biochem. Physiol. 68:232–240, 2008. © 2008 Wiley‐Liss, Inc.