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Pyridalyl inhibits cellular protein synthesis in insect, but not mammalian, cell lines
Author(s) -
Moriya Koko,
Hirakura Setsuko,
Kobayashi Jun,
Ozoe Yoshihisa,
Saito Shigeru,
Utsumi Toshihiko
Publication year - 2008
Publication title -
archives of insect biochemistry and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.576
H-Index - 66
eISSN - 1520-6327
pISSN - 0739-4462
DOI - 10.1002/arch.20252
Subject(s) - cytotoxicity , sf9 , cell culture , spodoptera , biology , protein biosynthesis , cell , biochemistry , spodoptera litura , insect , in vitro , toxicology , larva , gene , botany , genetics , recombinant dna
To gain insight into the mechanism of action and selectivity of the insecticidal activity of pyridalyl, the cytotoxicity of pyridalyl against various insect and mammalian cell lines was characterized by measuring the inhibition of cellular protein synthesis. When the effect of pyridalyl on the cellular protein synthesis in Sf9 cells was evaluated by measuring the incorporation of [ 3 H]leucine, rapid and significant inhibition of protein synthesis was observed. However, pyridalyl did not inhibit protein synthesis in a cell‐free protein synthesis system, indicating that pyridalyl does not directly inhibit protein synthesis. No obvious cytotoxicity was observed against any of the mammalian cell lines tested. In the case of insect cell lines, remarkable differences in the cytotoxicity of pyridalyl were observed: the highest cytotoxicity (IC 50 mM) was found against Sf9 cells derived from Spodoptera frugiperda , whereas no obvious cytotoxicity was observed against BmN4 cells derived from Bombyx mori . Measurements of the insecticidal activity of pyridalyl against Spodoptera litura and B. mori revealed a correlation between the cytotoxicity against cultured cell lines and the insecticidal activity. From these observations, it was concluded that the selective inhibition of cellular protein synthesis by pyridalyl might contribute significantly to the insecticidal activity and the selectivity of this compound. Arch. Insect Biochem. Physiol. 2008. © 2008 Wiley‐Liss, Inc.