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Prostaglandin E 2 modulates the expression of antimicrobial peptides in the fat body and midgut of Anopheles albimanus
Author(s) -
García Gil de Muñoz F.L.,
MartínezBarnetche J.,
LanzMendoza H.,
Rodríguez M.H.,
HernándezHernández F.C.
Publication year - 2008
Publication title -
archives of insect biochemistry and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.576
H-Index - 66
eISSN - 1520-6327
pISSN - 0739-4462
DOI - 10.1002/arch.20232
Subject(s) - midgut , biology , anopheles albimanus , cecropin , antimicrobial peptides , malpighian tubule system , prostaglandin e2 , arachidonic acid , micrococcus luteus , prostaglandin e , antimicrobial , microbiology and biotechnology , biochemistry , endocrinology , anopheles , immunology , escherichia coli , enzyme , gene , larva , botany , malaria
Prostaglandins (PGs) participate in the regulation of vertebrate and in at least six insect orders' immune responses. We identified PGE 2 in midgut, fat body, Malpighian tubules, and ovarioles of Anopheles albimanus ( Aa ) mosquitoes. Our data indicate that PGE 2 synthesis in cultured midguts responds to the presence of two bacterial species, Micrococcus luteus and Klebsiella pneumoniae . The production of mRNA coding for antimicrobial peptides Aa ‐Attacin, Aa ‐Cecropin, and Aa ‐Gambicin was observed in cultured fat bodies and midguts. The production of these messengers was reduced in the presence of dexamethasone, and this effect was reversed by arachidonic acid. Adding PGE 2 to cultures resulted in increased Aa ‐cecropin mRNA and decreased Aa ‐attacin and Aa ‐gambicin mRNAs. Arch. Insect Biochem. Physiol. 68:14–25, 2008. © 2008 Wiley‐Liss, Inc.