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cDNA cloning, characterization, and developmental expression of the 20S proteasome α5 subunit in the Mediterranean fruit fly Ceratitis capitata
Author(s) -
Verras Meletios,
Gourzi Polyxeni,
Kalosaka Katerina,
Zacharopoulou Antigone,
Mintzas Anastassios C.
Publication year - 2008
Publication title -
archives of insect biochemistry and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.576
H-Index - 66
eISSN - 1520-6327
pISSN - 0739-4462
DOI - 10.1002/arch.20226
Subject(s) - biology , ceratitis capitata , complementary dna , drosophila melanogaster , microbiology and biotechnology , protein subunit , northern blot , coding region , amino acid , messenger rna , homology (biology) , gene , genetics , tephritidae , botany , pest analysis
In the present study, we report the cDNA cloning, characterization, and developmental expression of the 20S proteasome α5 subunit from the Mediterranean fruit fly Ceratitis capitata (medfly). Using an RT‐PCR fragment that corresponds to the amino‐terminal region of the Drosophila melanogaster 20S proteasome α5 subunit, we isolated a 987‐bp cDNA that encodes the complete coding region of the medfly ortholog, which was named CcPSMA5. CcPSMA5 consists of 241 amino acids and has a predicted molecular weight of 26.4 kDa and pI 4.75. Comparison of the CcPSMA5 amino acid sequence with the sequences of all known 20S proteasome α5 subunits from different organisms indicated that the medfly 20S proteasome α5 subunit has the strongest homology to that of Drosophila . In situ hybridization showed that the CcPSMA5 gene is mapped in the region 44B of chromosome 4. Northern blot hybridization analysis showed that the CcPSMA5 mRNA has a size of approximately 1.2 kb. High levels of the CcPSMA5 mRNA were detected in freshly laid eggs, indicating that they were maternally deposited. The mRNA expression pattern during medfly development suggests that the CcPSMA5 gene is upregulated before mid‐embryogenesis and at the onset of metamorphosis. Arch. Insect Biochem. Physiol. © 2007 Wiley‐Liss, Inc.