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The DNA puff 4C expresses a salivary secretion protein in Trichosia pubescens (Diptera; Sciaridae)
Author(s) -
Andrioli Luiz Paulo,
Gorab Eduardo,
Amabis José Mariano
Publication year - 2008
Publication title -
archives of insect biochemistry and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.576
H-Index - 66
eISSN - 1520-6327
pISSN - 0739-4462
DOI - 10.1002/arch.20222
Subject(s) - biology , gene , complementary dna , dna , microbiology and biotechnology , polytene chromosome , southern blot , genomic dna , salivary gland , transcription (linguistics) , recombinant dna , genetics , drosophila melanogaster , biochemistry , linguistics , philosophy
DNA puffs are genomic regions of polytene chromosomes that undergo developmentally controlled DNA amplification and transcription in salivary glands of sciarid flies. Here, we tested the hypothesis that DNA puff genes code for salivary proteins in Trichosia pubescens . To do that, we generated antibodies against saliva and immunoscreened a cDNA library made from salivary glands. We isolated clones corresponding to DNA puff regions, including clone D‐50 that contained the entire coding sequence of the previously isolated C4B1 gene from puff 4C. Indeed, we showed that puff 4C is a DNA puff region detecting its local transcription and its extra rounds of DNA incorporation compared to neighboring regions. We further confirmed D‐50 clone identity in Western blots reacted with the anti‐saliva anitiserum. We detected a recombinant protein expressed by this clone that had the expected size for a full‐length product of the gene. We end with a discussion of the relationship between DNA puff genes and their products. Arch. Insect Biochem. Physiol. 2007. © 2007 Wiley‐Liss, Inc.