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Effects of the compounds 2‐methoxynaphthoquinone, 2‐propoxynaphthoquinone, and 2‐isopropoxynaphthoquinone on ecdysone 20‐monooxygenase activity
Author(s) -
Mitchell Martin J.,
Brescia Aaron I.,
Smith Stan L.,
Morgan E. David
Publication year - 2007
Publication title -
archives of insect biochemistry and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.576
H-Index - 66
eISSN - 1520-6327
pISSN - 0739-4462
DOI - 10.1002/arch.20196
Subject(s) - ecdysone , biology , manduca sexta , midgut , 20 hydroxyecdysone , instar , monooxygenase , insect , aedes aegypti , ecdysteroid , larva , biochemistry , botany , enzyme , cytochrome p450
The effects of the natural compound 2‐methoxy‐1,4‐naphthoquinone, isolated from the leaves of Impatiens glandulifera and the synthetic compounds 2‐propoxy‐1,4‐naphthoquinone and 2‐isopropoxy‐1,4‐naphthoquinone on ecdysone 20‐monooxygenase (E‐20‐M) activity were examined in three insect species. Homogenates of wandering stage third instar larvae of Drosophila melanogaster , or abdomens from adult female Aedes aegypti , or fat body or midgut from fifth instar larvae of Manduca sexta were incubated with radiolabelled ecdysone and increasing concentrations (from 1 × 10 –8 to 1 × 10 –3 M) of the three compounds. All three compounds were found to inhibit in a dose‐dependent fashion the E‐20‐M activity in the three insect species. The concentration of these compounds required to elicit a 50% inhibition of this steroid hydroxylase activity in the three insect species examined ranged from approximately 3 × 10 –5 to 7 × 10 –4 M. Arch. Insect Biochem. Physiol. 66:45–52, 2007. © 2007 Wiley‐Liss, Inc.