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Significance of the 19‐kDa hemolymph protein HP19 for the development of the rice moth Corcyra cephalonica : morphological and biochemical effects caused by antibody application
Author(s) -
Arif Abul,
Gullipalli Damodar,
Scheller Klaus,
DuttaGupta Aparna
Publication year - 2007
Publication title -
archives of insect biochemistry and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.576
H-Index - 66
eISSN - 1520-6327
pISSN - 0739-4462
DOI - 10.1002/arch.20195
Subject(s) - hemolymph , biology , ecdysteroid , 20 hydroxyecdysone , in vivo , polyclonal antibodies , juvenile hormone , metamorphosis , antibody , larva , biochemistry , manduca sexta , in vitro , hormone , botany , immunology , microbiology and biotechnology
The hemolymph protein HP19 of the rice moth, Corcyra cephalonica , mediates the 20‐hydroxyecdysone (20E)‐dependent acid phosphatase (ACP) activity at a nongenomic level. Affinity‐purified polyclonal antibody against HP19 (αHP19‐IgG) was used in the present study to understand the role of HP19 during the postembryonic development of Corcyra . In the in vitro studies, HP19 action was blocked either by immuno‐precipitation using αHP19‐IgG, prior to its addition to the fat body culture or by the addition of the antibody directly to the culture, along with 20E and hemolymph containing HP19. The αHP19‐IgG blocked the HP19‐mediated 20E‐dependent ACP activation. In the in vivo studies, the αHP19‐IgG was injected into the fully developed last (final/Vth) instar larvae of Corcyra , to complex the HP19 in vivo, in order to block the action of HP19. The injection of αHP19‐IgG resulted in defective development of larvae, which grew either into non‐viable larvae or larval‐pupal/pupal‐adult intermediates relative to the effect of pre‐immune IgG injected controls. The present study shows that HP19 plays an important role in controlling the metamorphosis of Corcyra by regulating the 20E‐dependent ACP activity. Coupled with the earlier findings, the ecdysteroid hormone regulates this action at a nongenomic level. Arch. Insect Biochem. Physiol. 66:32–44, 2007. © 2007 Wiley‐Liss, Inc.