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Identification of a putative azadirachtin‐binding complex from Drosophila Kc167 cells
Author(s) -
Robertson Susan L.,
Ni Weiting,
Dhadialla Tarlochan S.,
Nisbet Alasdair J.,
McCusker Catherine,
Ley Steven V.,
Mordue William,
Mordue Luntz A. Jennifer
Publication year - 2007
Publication title -
archives of insect biochemistry and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.576
H-Index - 66
eISSN - 1520-6327
pISSN - 0739-4462
DOI - 10.1002/arch.20171
Subject(s) - azadirachta , azadirachtin , biology , drosophila (subgenus) , hsp60 , drosophila melanogaster , heat shock protein , microbiology and biotechnology , biochemistry , gene , botany , hsp70 , pesticide , agronomy
Cell‐proliferation in Drosophila Kc167 cells was inhibited by 50% when cell cultures contained 1.7 × 10 –7 M azadirachtin for 48 h (a tertranortriterpenoid from the neem tree Azadirachta indica ). Drosophila Kc167 cells exhibited direct nuclear damage within 6‐h exposure to azadirachtin (5 × 10 –7 M and above) or within 24 h when lower concentrations were used (1 × 10 –9 M). Fractionation of an extract of Drosophila Kc167 cells combined with ligand overlay technique resulted in the identification of a putative azadirachtin binding complex. Identification of the members of this complex by Peptide Mass Fingerprinting (PMF) and N ‐terminal sequencing identified heat shock protein 60 (hsp60) as one of its components. Arch. Insect Biochem. Physiol. 64:200–208, 2007. © 2007 Wiley‐Liss, Inc.