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cDNA characterization and expression analysis of two arylphorin‐like hexameric protein genes from the diamondback moth, Plutella xylostella (L.)
Author(s) -
Ashfaq Muhammad,
Sonoda Shoji,
Tsumuki Hisaaki
Publication year - 2007
Publication title -
archives of insect biochemistry and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.576
H-Index - 66
eISSN - 1520-6327
pISSN - 0739-4462
DOI - 10.1002/arch.20168
Subject(s) - diamondback moth , biology , plutella , gene , complementary dna , amino acid , genetics , biochemistry , microbiology and biotechnology , lepidoptera genitalia , botany
We cloned and characterized two hexameric storage protein genes, PxAry1 and PxAry2 , from Plutella xylostella and investigated the expression pattern in different developmental stages and in response to treatment by a juvenile hormone (JH) analog. The complete coding sequences of PxAry1 and PxAry2 are comprised of 2,097 and 2,094 bp with 699 and 698 amino acid residues, respectively. Signal peptides of 16 amino acids are predicted at the N‐termini. According to both the phylogenetic analysis and amino acid composition (>16% aromatic amino acids), PxAry1 and PxAry2 belong to the arylphorin‐like protein genes. Analysis using Northern hybridization and RT‐PCR showed varying levels of genes expression in the developmental stages with a small difference between sexes. Expression of both genes in fourth instar larvae was suppressed after treatment with a JH‐analog. Southern hybridization revealed the presence of multiple arylphorin genes in the genome. Arch. Insect Biochem. Physiol. 64:175–185, 2007. © 2007 Wiley‐Liss, Inc.