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Identification of trf2 mutants of Drosophila with defects in anterior spiracle eversion
Author(s) -
Shima Seigo,
Aigaki Toshiro,
Nojima Tetsuya,
Yamamoto Daisuke
Publication year - 2007
Publication title -
archives of insect biochemistry and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.576
H-Index - 66
eISSN - 1520-6327
pISSN - 0739-4462
DOI - 10.1002/arch.20166
Subject(s) - biology , mutant , transcription factor , genetics , chromatin remodeling , locus (genetics) , chromatin , microbiology and biotechnology , gene
TATA‐box‐binding protein (TBP)‐related factor (Trf)2 is a member of the family of TBP‐related factors present in metazoan organisms. In Drosophila , Trf2 immunoprecipitates with the nucleosome remodeling factor (NURF) chromatin remodeling complex and the DNA replication element (DRE)‐binding factor DREF. When it forms a complex with DREF, Trf2 activates transcription from the DRE‐binding sites of the proliferating cell nuclear antigen (PCNA) gene. Despite these observations at the molecular level, no mutations in the trf2 locus have been found in Drosophila . Here, we identify two P‐element insertion alleles, PL28 and GS7403 , as hypomorphic mutants with a decreased expression level of trf2 . Pupae of these mutant alleles show failure in anterior spiracle eversion, a hallmark of mutations in the loci associated with ecdysteroid signaling. Arch. Insect Biochem. Physiol. 64:157–163, 2007. © 2007 Wiley‐Liss, Inc.