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Antioxidant defense in mitochondria during diapause and postdiapause development of European corn borer ( Ostrinia nubilalis , Hubn.)
Author(s) -
JovanovićGalović A.,
Blagojević D.P.,
GruborLajšić G.,
Worland M.R.,
Spasić M.B.
Publication year - 2007
Publication title -
archives of insect biochemistry and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.576
H-Index - 66
eISSN - 1520-6327
pISSN - 0739-4462
DOI - 10.1002/arch.20160
Subject(s) - ostrinia , european corn borer , biology , diapause , pyralidae , glutathione reductase , glutathione peroxidase , catalase , pupa , botany , lepidoptera genitalia , antioxidant , larva , biochemistry
Antioxidant enzymes (CAT, catalase; GPx, selenium nondependent glutathione peroxidase; GST, glutathione‐S‐transferase; GR, glutathione reductase; DHAR, dehydroascorbate reductase) were determined in the mitochondria of diapausing and non‐diapausing larvae and pupae of both diapausing and non‐diapausing larvae of the European corn borer ( Ostrinia nubilalis, Hubn ., Lepidoptera: Pyralidae). CAT, GST, and DHAR activity in mitochondria of diapausing larvae were reduced compared to non‐diapausing larvae. Pupae of diapaused‐larvae possessed lower GST, but higher DHAR activities compared to pupae of non‐diapaused individuals. Comparison between larvae and pupae revealed lower GPx activity in the mitochondria of pupae. CAT activity in the mitochondria of pupae was higher compared to diapausing larvae, but lower than in non‐diapausing ones. Correlation and canonical discriminant analyses revealed different antioxidant enzyme compositions for a particular stage and developmental pattern. Our results show that antioxidant enzymes have a similar role in the regulation of energetics in mitochondria as that in diapause and metamorphosis. Arch. Insect Biochem. Physiol. 64:111–119, 2007. © 2007 Wiley‐Liss, Inc.