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Growth and mitogenic effects of arylphorin in vivo and in vitro
Author(s) -
Hakim R.S.,
Blackburn M.B.,
Corti P.,
Gelman D.B.,
Goodman C.,
Elsen K.,
Loeb M.J.,
Lynn D.,
Soin T.,
Smagghe G.
Publication year - 2007
Publication title -
archives of insect biochemistry and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.576
H-Index - 66
eISSN - 1520-6327
pISSN - 0739-4462
DOI - 10.1002/arch.20155
Subject(s) - midgut , biology , manduca sexta , hemolymph , spodoptera littoralis , insect , sphingidae , cell growth , biochemistry , botany , microbiology and biotechnology , larva , noctuidae
In insects, developmental responses are organ‐ and tissue‐specific. In previous studies of insect midgut cells in primary tissue cultures, growth‐promoting and differentiation factors were identified from the growth media, hemolymph, and fat body. Recently, it was determined that the mitogenic effect of a Manduca sexta fat body extract on midgut stem cells of Heliothis virescens was due to the presence of monomeric α‐arylphorin. Here we report that in primary midgut cell cultures, this same arylphorin stimulates stem cell proliferation in the lepidopterans M. sexta and Spodoptera littoralis , and in the beetle Leptinotarsa decemlineata . Studies using S. littoralis cells confirm that the mitogenic effect is due to free α‐arylphorin subunits. In addition, feeding artificial diets containing arylphorin increased the growth rates of several insect species. When tested against continuous cell lines, including some with midgut and fat body origins, arylphorin had no effect; however, a cell line derived from Lymantria dispar fat body grew more rapidly in medium containing a chymotryptic digest of arylphorin. Arch. Insect Biochem. Physiol. 64:63–73, 2007. Published 2007 Wiley‐Liss, Inc.