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Use of the glucosidase inhibitor 1,4‐dideoxy‐1,4‐imino‐D‐arabinitol as a probe to investigate hypertrehalosemic hormone‐mediated change in carbohydrate and lipid metabolism in the cockroach, Periplaneta americana
Author(s) -
Oguri E.,
Steele J.E.
Publication year - 2006
Publication title -
archives of insect biochemistry and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.576
H-Index - 66
eISSN - 1520-6327
pISSN - 0739-4462
DOI - 10.1002/arch.20152
Subject(s) - periplaneta , hemolymph , cockroach , adipokinetic hormone , lipid metabolism , biology , phospholipid , trehalose , carbohydrate metabolism , endocrinology , medicine , biochemistry , metabolism , carbohydrate , hormone , fat body , membrane , gene , ecology
The glucosidase inhibitor 1,4‐dideoxy‐1,4‐imino‐D‐arabinitol (DIA) was used to investigate the action of hypertrehalosemic hormone (HTH) on carbohydrate, neutral lipid, and phospholipid metabolism in the hemolymph and fat body of the cockroach, Periplaneta americana . DIA blocked the hypertrehalosemic and hyperglycemic effects of HTH, as well as the decrease in hemolymph neutral lipid and phospholipid normally induced by HTH. DIA diminished the accumulation of neutral lipid in the fat body formed under the influence of HTH and partly blocked the decrease in fat body phospholipid evoked by HTH. The increased incorporation of 14 C‐glucose into fat body triacylglycerol in the presence of HTH was decreased by more than two‐thirds when DIA was coinjected with the hormone. The results suggest that glucose derived from hemolymph trehalose is an important contributor to the formation of the glycerol backbone of newly formed triacylglycerol in the fat body. Arch. Insect Biochem. Physiol. 63:169–176, 2006. © 2006 Wiley‐Liss, Inc.