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Induction of DNA synthesis in Anopheles albimanus tissue cultures in response to a Saccharomyces cerevisiae challenge
Author(s) -
HernándezMartínez S.,
RománMartínez U.,
MartínezBarnetche J.,
Garrido E.,
Rodríguez M.H.,
LanzMendoza H.
Publication year - 2006
Publication title -
archives of insect biochemistry and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.576
H-Index - 66
eISSN - 1520-6327
pISSN - 0739-4462
DOI - 10.1002/arch.20150
Subject(s) - biology , anopheles albimanus , dna synthesis , microbiology and biotechnology , dna , zymosan , polytene chromosome , proliferating cell nuclear antigen , biochemistry , concanavalin a , saccharomyces cerevisiae , yeast , gene , immunology , in vitro , chromosome , anopheles , malaria
DNA synthesis was detected by the incorporation of 5‐bromo‐2′ deoxy‐uridine (BrdU) in adult Anopheles albimanus organs in culture in response to a challenge with Saccharomyces cerevisiae . Abdomens of mosquitoes inoculated with Roswell Park Memorial Institute medium (RPMI, control) or yeast were cultivated in RPMI plus ConA and BrdU for 5 days. DNA was obtained by phenolic extraction and the incorporated BrdU was quantified by ELISA using anti‐BrdU peroxidase‐labeled antibodies. Abdomen tissues of mosquitoes inoculated with yeast showed higher DNA synthesis than controls. Organs from untreated mosquitoes cultured in the presence of zymosan also synthesized DNA but at a lower level than tissues from yeast‐inoculated mosquitoes. In similar experiments, DNA synthesis was inhibited by the addition of colchicine. DNA synthesis, evidenced by epifluorescence using an anti‐BrdU fluorescein‐labeled antibody, occurred in fat body, epithelial cells in pleural membranes, and the dorsal vessel. Pleural membranes showed the highest number of labeled cells. These tissues were also labeled with anti‐PCNA (proliferating cell nuclear antigen) antibodies, two of which were able to produce polytene chromosomes under yeast stimulation. These results demonstrate that different An. albimanus tissues undergo DNA synthesis in response to foreign particles. Arch. Insect Biochem. Physiol. 63:147–158, 2006. © 2006 Wiley‐Liss, Inc.