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Comparative structure‐activity analysis of insect kinin core analogs on recombinant kinin receptors from Southern cattle tick Boophilus microplus (Acari: Ixodidae) and mosquito Aedes aegypti (Diptera: Culicidae)
Author(s) -
TanejaBageshwar Suparna,
Strey Allison,
Zubrzak Pawel,
Pietrantonio Patricia V.,
Nachman Ronald J.
Publication year - 2006
Publication title -
archives of insect biochemistry and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.576
H-Index - 66
eISSN - 1520-6327
pISSN - 0739-4462
DOI - 10.1002/arch.20129
Subject(s) - kinin , biology , pentapeptide repeat , aedes aegypti , receptor , biochemistry , peptide , botany , bradykinin , larva
The systematic analysis of structure‐activity relationships of insect kinins on two heterologous receptor‐expressing systems is described. Previously, kinin receptors from the southern cattle tick, Boophilus microplus (Canestrini) [Holmes et al., Insect Mol Biol 9:457–465 (2000); Holmes et al., Insect Mol Biol 12:27–38 (2003)], and the dengue vector, the mosquito Aedes aegypti (L.) [Pietrantonio et al., Insect Mol Biol 14:55–67 (2005)], were functionally and stably expressed in CHO‐K1 cells. In order to determine which kinin residues are critical for the peptide‐receptor interaction, kinin core analogs were synthesized as an Ala‐replacement series of the peptide FFSWGa and tested by a calcium bioluminescence plate assay. The amino acids Phe 1 and Trp 4 were essential for activity of the insect kinins in both receptors. It was confirmed that the pentapeptide kinin core is the minimum sequence required for activity and that the C‐terminal amide is also essential. In contrast to the tick receptor, a large increase in efficacy is observed in the mosquito receptor when the C‐terminal pentapeptide is N‐terminally extended to a hexapeptide. The aminoisobutyric acid (Aib)‐containing analog, FF[Aib]WGa, was as active as superagonist FFFSWGa on the mosquito receptor in contrast to the tick receptor where it was statistically more active than FFFSWGa by an order of magnitude. This restricted conformation Aib analog provides information on the conformation associated with the interaction of the insect kinins with these two receptors. Furthermore, the analog FF[Aib]WGa has been previously shown to resist degradation by the peptidases ACE and nephrilysin and represents an important lead in the development of biostable insect kinin analogs that ticks and mosquitoes cannot readily deactivate. Arch Insect Biochem Physiol 62:128–140, 2006. Published 2006 Wiley‐Liss, Inc.