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Cloning and nucleotide sequencing of three heat shock protein genes ( hsp90 , hsc70 , and hsp19.5 ) from the diamondback moth, Plutella xylostella (L.) and their expression in relation to developmental stage and temperature
Author(s) -
Sonoda Shoji,
Ashfaq Muhammad,
Tsumuki Hisaaki
Publication year - 2006
Publication title -
archives of insect biochemistry and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.576
H-Index - 66
eISSN - 1520-6327
pISSN - 0739-4462
DOI - 10.1002/arch.20124
Subject(s) - diamondback moth , biology , complementary dna , gene , heat shock protein , orfs , intron , microbiology and biotechnology , open reading frame , genetics , plutella , rapid amplification of cdna ends , genomic dna , molecular cloning , peptide sequence , botany , lepidoptera genitalia
Heat shock protein genes, hsp90 , hsc70 , and hsp19.5 , were cloned and sequenced from the diamondback moth, Plutella xylostella (L.) by RT‐PCR and RACE method. The cDNA sequence analysis of hsp90 and hsp19.5 revealed open reading frames (ORFs) of 2,151 and 522 bp in length, which encode proteins with calculated molecular weights of 82.4 and 19.5 kDa, respectively. Analysis of cDNA from hsc70 revealed an ORF of 1,878 bp coding a protein with a calculated molecular weight of 69.3 kDa. Furthermore, the analysis of genomic DNA from hsc70 confirmed the presence of introns while no introns were apparent in hsp90 and hsp19.5 . Southern blot analysis suggested the presence of multiple copies of each gene family in the DBM genome. Detectable expression of hsp19.5 was observed at the pupal stage while expression of hsp90 and hsc70 was detected at both pupal and adult stages. At adult stage, females showed a higher expression of hsp90 and hsc70 than males. An increased expression was observed in all three genes after exposure to a high temperature in both sexes. These results suggest that in addition to a heat shock response, these HSP genes might be involved in other functions during the course of development in DBM. Arch. Insect Biochem. Physiol. 62:80–90, 2006. © 2006 Wiley‐Liss, Inc.