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Molecular cloning and characterization of ATX1 cDNA from the mole cricket, Gryllotalpa orientalis
Author(s) -
Kim Iksoo,
Lee Kwang Sik,
Hwang Jae Sam,
Ahn Mi Young,
Yun Eun Young,
Li Jian Hong,
Sohn Hung Dae,
Jin Byung Rae
Publication year - 2006
Publication title -
archives of insect biochemistry and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.576
H-Index - 66
eISSN - 1520-6327
pISSN - 0739-4462
DOI - 10.1002/arch.20114
Subject(s) - biology , complementary dna , cdna library , microbiology and biotechnology , peptide sequence , rapid amplification of cdna ends , amino acid , molecular cloning , biochemistry , gene
To search for an insect homologue of antioxidant protein 1 (ATX1), a mole cricket, Gryllotalpa orientalis , cDNA library was screened and a cDNA clone, which encodes a 73 amino acid polypeptide with a predicted molecular mass of 8.0 kDa and pI of 5.68, was isolated. The G. orientalis ATX1 (GoATX1) cDNA features both a MTC XX C copper‐binding site in the N‐terminus and a KTGK lysine‐rich region in the C‐terminus. The deduced amino acid sequence of the GoATX1 cDNA showed 63% identity to Drosophila melanogaster ATX1 and 55% to Ixodes pacificus ATX1. Northern blot analysis revealed the presence of GoATX1 transcripts in midgut, fat body, and epidermis. When H 2 O 2 was injected into the body cavity of G. orientalis adult, GoATX1 mRNA expression was up‐regulated in the fat body tissue. Fat body expression level of GoATX1 mRNA in the fat body was increased following exposure to low (4°C) and high (37°C) temperatures, suggesting that GoATX1 plays a protective role against oxidative stress caused by temperature shock. This is the first report about a functional role of insect ATX1 in antioxidant defense. Arch. Insect Biochem. Physiol. 61:231–238, 2006. © 2006 Wiley‐Liss, Inc.