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Characteristics of two genes encoding proteins with an ADAM‐type metalloprotease domain, which are induced during the molting periods in Bombyx mori
Author(s) -
Ote Manabu,
Mita Kazuei,
Kawasaki Hideki,
Kobayashi Masahiko,
Shimada Toru
Publication year - 2005
Publication title -
archives of insect biochemistry and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.576
H-Index - 66
eISSN - 1520-6327
pISSN - 0739-4462
DOI - 10.1002/arch.20059
Subject(s) - disintegrin , biology , ecdysis , thrombospondin , bombyx mori , metalloproteinase , gene , moulting , bombyx , microbiology and biotechnology , hemolymph , extracellular matrix , genetics , matrix metalloproteinase , biochemistry , larva , botany
Abstract By microarray analyses, we identified two genes ( BmADAMTS‐1 and BmADAMTS‐like ) encoding a protein, which are induced during the pupal ecdysis in the wing discs of Bombyx mori ; these genes are homologous to ADAMTS family members (a disintegrin and metalloproteinase domain, with thrombospondin type‐1 repeats). A complete metal‐binding motif of the ADAM‐type metalloprotease domain (HEXXHXXGXXHD) was contained in both amino acid sequences. However, thrombospondin type 1 (TSP‐1) repeats were observed only in BmADAMTS‐1. The BmADAMTS‐1 gene was expressed in the hemocyte and midgut of the larvae at day 2 of wandering stage (W2), and strongly induced during the pupal ecdysis in the hemolymph. The BmADAMTS‐like gene was expressed in the epithelial tissues of the larvae at W2, and had expression peaks slightly later than the BmADAMTS‐1 gene. Our results indicate that BmADAMTS‐1 and BmADAMTS‐like may cleave the extracellular matrix (ECM) in the degenerating and remodeling tissues during the molting periods. Arch. Insect Biochem. Physiol. 59:91–98, 2005. © 2005 Wiley‐Liss, Inc.