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Glutathione S‐transferases in the adaptation to plant secondary metabolites in the Myzus persicae aphid
Author(s) -
Francis Frédéric,
Vanhaelen Nicolas,
Haubruge Eric
Publication year - 2005
Publication title -
archives of insect biochemistry and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.576
H-Index - 66
eISSN - 1520-6327
pISSN - 0739-4462
DOI - 10.1002/arch.20049
Subject(s) - myzus persicae , allelopathy , biology , isozyme , brassica , glutathione , aphid , brevicoryne brassicae , brassicaceae , biochemistry , enzyme , metabolite , botany , germination , pest analysis , aphididae , homoptera
Abstract Glutathione S‐transferases (GST) in insects play an important role in the detoxification of many substances including allelochemicals from plants. Induction of GST activity in Myzus persicae in response to secondary metabolites from Brassica plants was determined using different host plant species and confirmed using artificial diet with pure allelochemicals added. The 2,4‐dinitro‐1‐iodobenzene (DNIB) was found to be a useful substrate for identifying particular GSTs in insects. GSTs from M. persicae were purified using different affinity chromatography columns and related kinetic parameters were calculated. GST isoenzymes were characterised using electrophoretic methods. Although SDS‐PAGE results indicated similarity among the purified enzymes from each affinity column, biochemical studies indicated significant differences in kinetic parameters. Finally, the GST pattern of M. persicae was discussed in terms of insect adaptation to the presence of plant secondary substances such as the glucosinolates and the isothiocyanates, from Brassicaceae host plants. Arch. Insect Biochem. Physiol. 58:166–174, 2005. © 2005 Wiley‐Liss, Inc.