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Male accessory gland derived factors can stimulate oogenesis and enhance oviposition in Helicoverpa armigera (Lepidoptera: Noctuidae)
Author(s) -
Jin ZhaoYang,
Gong He
Publication year - 2001
Publication title -
archives of insect biochemistry and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.576
H-Index - 66
eISSN - 1520-6327
pISSN - 0739-4462
DOI - 10.1002/arch.1027
Subject(s) - noctuidae , lepidoptera genitalia , biology , helicoverpa armigera , oogenesis , fractionation , mating , bioassay , sex pheromone , pheromone , insect , oocyte , botany , andrology , anatomy , zoology , chromatography , ecology , microbiology and biotechnology , embryo , medicine , chemistry
Abstract In Helicoverpa armigera , female moths began to lay eggs on the third day after emergence. Mating stimulated earlier egg maturation/oogenesis ( P = 0.002) and oviposition ( P << 0.01). We established a suitable bioassay model for the influence of male accessory glands (MAG) on the physiology of virgin females: Crude extracts of MAG (2‐ to 3‐day‐old) were injected into 2‐day‐old virgin females, and the injected females were dissected 20 h after mating. It was shown that crude extracts of MAG stimulated earlier egg maturation ( P < 0.001) and oviposition (the oviposition ratio was more than 2 times the ratio of the control). Proteinaceous components in crude extracts purified by fractionation and sub‐fractionation in reverse phase high performance liquid chromatography also stimulated earlier egg maturation ( P < 0.01) and ovipositon (more than 2 times the ratio of the control), and we called them the oogenesis and ovipostion factors (OOSF). With SDS‐PAGE, the molecular mass of the bands from OOSF was estimated to be between 55–66 KD. Arch. Insect Biochem. Physiol. 46:175–185, 2001. © 2001 Wiley‐Liss, Inc.