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Stimulation of midgut stem cell proliferation by Manduca sexta α‐arylphorin
Author(s) -
Blackburn Michael B.,
Loeb Marcia J.,
Clark Edward,
Jaffe Howard
Publication year - 2004
Publication title -
archives of insect biochemistry and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.576
H-Index - 66
eISSN - 1520-6327
pISSN - 0739-4462
DOI - 10.1002/arch.10119
Subject(s) - manduca sexta , biology , midgut , sphingidae , stimulation , microbiology and biotechnology , manduca , stem cell , cell growth , biochemistry , botany , neuroscience , insect , larva
Extracts of the green‐colored perivisceral fat body of newly ecdysed Manduca sexta pupae stimulate mitosis in midgut stem cells of Heliothis virescens cultured in vitro. Using a combination of cation‐ and anion‐exchange chromatography, we have isolated a protein from these fat body extracts that accounts for the observed stem cell proliferation. SDS‐PAGE analysis of the protein results in a single band of 77 kDa. Sequences of tryptic peptides from this protein are identical to internal sequences of the storage hexamer α‐arylphorin. The α‐arylphorin isolated by our procedure represents a small fraction of the total arylphorin present in the fat body extract. However, it alone seems responsible for the stimulation of mitotic activity in H. virescens midgut stem cells. Arch. Insect Biochem. Physiol. 55:26–32, 2004. Published 2003 Wiley‐Liss, Inc.