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Cholinergic regulation of the corpora allata in adult male loreyi leafworm Mythimna loreyi
Author(s) -
Kou Rong
Publication year - 2002
Publication title -
archives of insect biochemistry and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.576
H-Index - 66
eISSN - 1520-6327
pISSN - 0739-4462
DOI - 10.1002/arch.10022
Subject(s) - endocrinology , muscarinic antagonist , medicine , carbachol , pirenzepine , muscarinic acetylcholine receptor , acetylcholine , antagonist , cholinergic , corpus allatum , physostigmine , biology , juvenile hormone , receptor , hormone , stimulation
The direct effect of acetylcholine on the activation of the corpora allata (CA) was investigated in the adult male loreyi leafworm, Mythimna loreyi . Acetylcholine, in the presence of the choline esterase inhibitor physostigmine (50 μM), elicited a stimulatory effect on juvenile hormone acids (JHAs) release from the CA. Maximum effect was obtained at concentrations of 10 and 50 μM. Repeated administration of 10 μM acetylcholine on the same CA did not elicit similar stimulatory effect. Since JHA release can be significantly activated by carbachol and not by nicotine, this cholinergic effect is likely to belong to the muscarinic type. The effect of acetylcholine was significantly antagonized by gallamine triethiodide (M 2 antagonist) and 4‐DAMP (M 3 antagonist), pirenzepine (M 1 antagonist), and tropicamide (M 4 antagonist) were ineffective. It is concluded that in the adult male M. loreyi , the cholinergic regulation of CA is most likely via M 2 and M 3 muscarinic receptors.Arch. Insect Biochem. Physiol. 49:215–224, 2002. © 2002 Wiley‐Liss, Inc.